Modulation of Oxidative Stress Biomarkers by Ascorbic Acid and Creatine Co-Treatment in Male Rabbits”

Oxidative stress plays a central role in the pathogenesis of numerous metabolic and degenerative diseases. Antioxidants such as ascorbic acid and creatine (CrS) have been shown to modulate oxidative damage, but limited research has investigated their combined effects in vivo. This study aimed to evaluate the effect of ascorbic acid, creatine, and their co-administration on oxidative stress biomarkers in male rabbits, focusing on catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and thiobarbituric acid-reactive substances (TBARS). Twenty male rabbits were randomly divided into four groups (n = 5): control, ascorbic acid (20 mg/kg), creatine 5 mg/kg), and a combination of both agents. Treatments were administered orally for 6weeks. Blood samples were analyzed for CAT, SOD, GSH, and TBARS using standard spectrophotometric methods. Data were statistically analyzed using one-way ANOVA followed by Tukey’s post hoc test. The co-treatment group showed a significant increase in CAT (1.12 ± 0.043 U/min/ml) and SOD (1.364 ± 0.040 U/ml) activities compared to the control (p < 0.05), suggesting enhanced enzymatic antioxidant defense. TBARS levels, an indicator of lipid peroxidation, were significantly reduced in all treated groups, with the lowest level in the ascorbic acid group (3.209 ± 0.051). GSH levels remained stable across all groups without significant variation (p = 0.882), indicating preservation of intracellular antioxidant capacity. ANOVA confirmed significant differences for CAT, SOD, and TBARS (p = 0.000), while GSH showed no significant change.